Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing the Panax has paved way for in-depth proteomic studies this medicinal plant. current study was conducted deepen information on root proteome Korean ginseng. Proteomic workflow optimized by testing two different strategies, characterized phenol extraction procedure, presence or absence SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. results highlighted an evident improvement combination with before nLC-MS/MS In addition, a dramatic impact steaming process (the treatment produce red from ginseng) protein properties observed. Overall, analyses permitted characterization total 2412 proteins. A large number identified proteins belonged functional categories carbon/energy metabolism (22.4% 14.6%, respectively). primary secondary metabolisms are major metabolic pathways, which emerged known play important role in response (a)biotic stresses were also identified. not only confirmed previous transcriptomic reports but extended information, including main pathways involved